Difference between revisions of "Part:BBa K313010:Experience"
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<br>[[image:SYSU-CHINA circRNA-2.png |border|center|300px]]</br>
 
<br>[[image:SYSU-CHINA circRNA-2.png |border|center|300px]]</br>
 
<br><font size="1"><center>Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab</center></font></br>
 
<br><font size="1"><center>Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab</center></font></br>
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===split EGFP===
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experimental data
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<br>We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.</br>

Revision as of 10:13, 12 October 2021


We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQ2381d596a07b431a-partinfo-00000000-QINU UNIQ2381d596a07b431a-partinfo-00000001-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

MS2 aptamer on the circRNA scaffold.png
Figure 1.2D stucture predicted by RNAfold

SYSU-CHINA circRNA-1.png
Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQ2381d596a07b431a-partinfo-00000002-QINU UNIQ2381d596a07b431a-partinfo-00000003-QINU
We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:


SYSU-CHINA circRNA-2.png
</br>
Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab
</br>

split EGFP

UNIQ2381d596a07b431a-partinfo-00000004-QINU UNIQ2381d596a07b431a-partinfo-00000005-QINU
We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.</br>