Difference between revisions of "Part:BBa K3887002"
 
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<partinfo>BBa_K3887002 short</partinfo>
 
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tryptophan 7-halogenase
  
 
=Description=
 
=Description=

Latest revision as of 07:52, 12 October 2021


RebH

tryptophan 7-halogenase

Description

RebH is a type of 7-halogenase enzyme used also during conversion of indole.

In our project, it is used to biosynthesis other types of indigo using Trp, similar to SttH producing Tyrian Purple. It is applied with the Fre-L3-halogenasee fusion technology.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 977
    Illegal XhoI site found at 891
    Illegal XhoI site found at 1537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1423
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 958
    Illegal BsaI.rc site found at 1540