Difference between revisions of "Part:BBa K3930025"

 
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<partinfo>BBa_K3930025 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930025 SequenceAndFeatures</partinfo>
  
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This cassette allows the selection of Yeast transformant upon addition of G418 into the media of culture. The sequence of the gene carrying the resistance is codon optimized for an expression into S.cerevisiae.
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<h2>Introduction</h2>
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<p>The NsrR cassette is composed by the promoter TEF1, the resistance gene <i>nsrR</i> and the transcription terminator of CYC1. The resistance gene is based on the part (BBa_K3629011), but it was codon optimized for expression into <i>S.cerevisiae</i>.</p>
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<p>This gene codes for a Nourseothricin acetyltransferase , which inactivate the nourseothricin by adding a acetyl on it. Therefore, this cassette aload the selection of Yeast transformant upon addition of nourseothricin into the media of culture. </p>
  
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<h3>Part characterization</h3>
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<p> The part (BBa_K3930025) was used to select the right genomic integration of the part (BBa_K3930003). Transformants were plated onto YPD + 250 &mu;g.ml-1 nourseothricin Petri dish. The Toulouse INSA UPS team manage to select a transformant with this selection marker (for more details, check the (BBa_K3930003) part page) </p>
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3930025 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 06:59, 12 October 2021


Nourseothricin selective cassette Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 710
    Illegal AgeI site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168

Introduction

The NsrR cassette is composed by the promoter TEF1, the resistance gene nsrR and the transcription terminator of CYC1. The resistance gene is based on the part (BBa_K3629011), but it was codon optimized for expression into S.cerevisiae.

This gene codes for a Nourseothricin acetyltransferase , which inactivate the nourseothricin by adding a acetyl on it. Therefore, this cassette aload the selection of Yeast transformant upon addition of nourseothricin into the media of culture.

Part characterization

The part (BBa_K3930025) was used to select the right genomic integration of the part (BBa_K3930003). Transformants were plated onto YPD + 250 μg.ml-1 nourseothricin Petri dish. The Toulouse INSA UPS team manage to select a transformant with this selection marker (for more details, check the (BBa_K3930003) part page)