Difference between revisions of "Part:BBa K3796203"
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===Characterization===
 
===Characterization===
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This year, our team designed a kill switch in Corynebacterium glutamicum using ndoA from Bacillus subtilis subsp. subtilis str. 168. We aimed to test if the overexpression of ndoA in Corynebacterium glutamicum can kill the enginnered bacteria effectively, and documented our works and failures in iGEM Parts to inspire future iGEM teams for kill switch design.
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This year, our team designed a kill switch in <i>Corynebacterium glutamicum </i> using <i>ndoA</i> from <i>Bacillus subtilis</i> subsp. subtilis str. 168. We aimed to test if the overexpression of <i>ndoA</i> in <i>Corynebacterium glutamicum</i> can kill the enginnered bacteria effectively, and documented our works and failures in iGEM Parts to inspire future iGEM teams for kill switch design.

Revision as of 14:25, 11 October 2021


ndoA (endoribonuclease toxin in Bacillus subtilis)

This coding region ndoA(also mazF, ydcE) encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system. It encodes endoribonuclease toxin in Bacillus subtilis that is a UACAU‐specific mRNA interferase, cleavaging after the first U. Previous research has proved that its overexpression is toxic for cell growth and it functions as normal in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This year, our team designed a kill switch in Corynebacterium glutamicum using ndoA from Bacillus subtilis subsp. subtilis str. 168. We aimed to test if the overexpression of ndoA in Corynebacterium glutamicum can kill the enginnered bacteria effectively, and documented our works and failures in iGEM Parts to inspire future iGEM teams for kill switch design.