Difference between revisions of "Part:BBa K3745030"
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The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.
 
The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.
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It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.
  
 
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Revision as of 12:38, 11 October 2021


Plac+rhlABC linear fragment

The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.

It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3524
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2149
    Illegal NgoMIV site found at 2458
    Illegal NgoMIV site found at 4067
    Illegal AgeI site found at 3070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4206