Difference between revisions of "Part:BBa K3745030:Design"
Jiaqi Tang (Talk | contribs) |
Jiaqi Tang (Talk | contribs) |
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===Design Notes=== | ===Design Notes=== | ||
− | + | It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI. | |
Revision as of 12:35, 11 October 2021
Plac+rhlABC linear fragment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3524
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2185
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2458
Illegal NgoMIV site found at 4067
Illegal AgeI site found at 3070 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 4206
Design Notes
It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.
Source
This study