Difference between revisions of "Part:BBa K4040020"

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<partinfo>BBa_K4040020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4040020 SequenceAndFeatures</partinfo>
 
===Usage and Biology===
 
===Usage and Biology===
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Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. And when the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed.  Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.  
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Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. We used CMV as the promoter. When the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed.  Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.  
  
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[[File:T--NMU_China--gp130-tev.jpg|400px|thumb|center|<b>Figure1.</b>Vector maps of tested CAR designs and schematics showing the structures of CARs.]]
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[[File:T--NMU_China--gp130-tev.jpg|300px|thumb|left|<b>Figure1.</b>Vector maps of tested CAR designs and schematics showing the structures of CARs.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:39, 11 October 2021


Synthetic Receptor GP130-TEV

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. We used CMV as the promoter. When the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed.  Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.  

Figure1.Vector maps of tested CAR designs and schematics showing the structures of CARs.