Difference between revisions of "Part:BBa K3745030"

Revision as of 10:58, 11 October 2021

Plac+rhlABC linear fragment

The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 3524
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2185
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2458
Illegal NgoMIV site found at 4067
Illegal AgeI site found at 3070
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 4206

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3745030 short</partinfo>
-The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli
+The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.
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