Difference between revisions of "Part:BBa K313010:Experience"

Revision as of 09:50, 11 October 2021

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQ097bf9735c52151e-partinfo-00000000-QINU UNIQ097bf9735c52151e-partinfo-00000001-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

Figure 1.2D stucture predicted by RNAfold

Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQ097bf9735c52151e-partinfo-00000002-QINU UNIQ097bf9735c52151e-partinfo-00000003-QINU We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:

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-We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:
+<br>We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:
 [[Image:MS2_aptamer_on_the_circRNA_scaffold.png ‎ | border | center | 300px]]
 <font size="1"><center>Figure 1.2D stucture predicted by RNAfold</center></font><br/>