Difference between revisions of "Part:BBa K3779009:Experience"

Revision as of 12:15, 10 October 2021

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3779009

① Codon optimization The original target gene BBa_K3779022 was codon optimized according to pichia pastoris preference, and the optimized codon SS-bgly (BBa_K3779001) was used for plasmid construction to improve the activity of our enzyme. ②the signal peptide We added the signal peptide α-factor (BBa_K3779002) to our plasmid, which contributed to the extracellular expression of our target protein in pichia pastoris. ③ AOX1 promoter We used methanol-induced promoters and designed 5 'and 3' primers BBa_K3779000 and BBa_K3779003. Integration of foreign genes ④ Integration of foreign genes Linearized plasmids were introduced into p. Pastoris genome for homologous integration by electrotransformation. This method is simple and efficient in practice.

User Reviews

UNIQe9c5b7164bed3594-partinfo-00000000-QINU UNIQe9c5b7164bed3594-partinfo-00000001-QINU

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 ===Applications of BBa_K3779009===
+① Codon optimization
+The original target gene BBa_K3779022 was codon optimized according to pichia pastoris preference, and the optimized codon SS-bgly (BBa_K3779001) was used for plasmid construction to improve the activity of our enzyme.
+②the signal peptide
+We added the signal peptide α-factor (BBa_K3779002) to our plasmid, which contributed to the extracellular expression of our target protein in pichia pastoris.
+③ AOX1 promoter
+We used methanol-induced promoters and designed 5 'and 3' primers BBa_K3779000 and BBa_K3779003.
+Integration of foreign genes
+④ Integration of foreign genes
+Linearized plasmids were introduced into p. Pastoris genome for homologous integration by electrotransformation. This method is simple and efficient in practice.
 ===User Reviews===