Difference between revisions of "Part:BBa K3791013:Design"

 
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===Design Notes===
 
===Design Notes===
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The repeat sequence must be placed before the spacer sequence, that is, in the 5’ extreme.
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===Source===
 
===Source===
  
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The repeat and the T7 promoter sequences were extracted from the parts registry ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2927006 BBa_K2927006]) ([https://parts.igem.org/Part:BBa_K1614000 BBa_K1614000]), whereas the spacer one was obtained from being the complementary sequence of an kanamycin-resistant gene DNA fragment. (as explained in its own part page: [https://parts.igem.org/Part:BBa_K3791000 BBa_K3791003]).
  
 
===References===
 
===References===
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[1] New England Biolabs. (s/f). <b>How do I design a guide RNA for use with EnGen Lba Cas12a?</b> Neb.com. From: https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a

Revision as of 12:00, 10 October 2021


gRNA Kanamycin construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The repeat sequence must be placed before the spacer sequence, that is, in the 5’ extreme.

Source

The repeat and the T7 promoter sequences were extracted from the parts registry (BBa_K2927006) (BBa_K1614000), whereas the spacer one was obtained from being the complementary sequence of an kanamycin-resistant gene DNA fragment. (as explained in its own part page: BBa_K3791003).

References

[1] New England Biolabs. (s/f). How do I design a guide RNA for use with EnGen Lba Cas12a? Neb.com. From: https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a