Difference between revisions of "Part:BBa K3791007"

 
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<span class='h3bb'>Sequence and Features</span>
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Revision as of 00:48, 10 October 2021


gRNA Erythromycin

Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (BBa_K2927006) and a spacer sequence which is specific for the desired gene to target (BBa_K3791002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]