Difference between revisions of "Part:BBa K3873999:Experience"
QinwenWang (Talk | contribs) (→Applications of BBa_K3873999) |
QinwenWang (Talk | contribs) (→Applications of BBa_K3873999) |
||
| Line 7: | Line 7: | ||
We used 0g/L, 0.001g/L and 1g/L tryptamine solution to induce the expression of oxytocin in the engineering bacteria and detected the intracellular and extracellular levels of oxytocin at 6 h respectively by Elisa. | We used 0g/L, 0.001g/L and 1g/L tryptamine solution to induce the expression of oxytocin in the engineering bacteria and detected the intracellular and extracellular levels of oxytocin at 6 h respectively by Elisa. | ||
| + | |||
The results showed that the expression level of oxytocin increased with time. In addition, the higher the induced concentration is, the more oxytocin will be expressed. | The results showed that the expression level of oxytocin increased with time. In addition, the higher the induced concentration is, the more oxytocin will be expressed. | ||
| + | |||
Moreover, the amount of intracellular oxytocin is lower than the amount of extracellular oxytocin, indicating that our signal peptide does play a role. | Moreover, the amount of intracellular oxytocin is lower than the amount of extracellular oxytocin, indicating that our signal peptide does play a role. | ||
Revision as of 18:09, 9 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3873999
We used 0g/L, 0.001g/L and 1g/L tryptamine solution to induce the expression of oxytocin in the engineering bacteria and detected the intracellular and extracellular levels of oxytocin at 6 h respectively by Elisa.
The results showed that the expression level of oxytocin increased with time. In addition, the higher the induced concentration is, the more oxytocin will be expressed.
Moreover, the amount of intracellular oxytocin is lower than the amount of extracellular oxytocin, indicating that our signal peptide does play a role.
Based on the above experimental results, we wanted to further explore the changes in the expression level of oxytocin over time and find the most suitable concentration of tryptophan to induce the expression. By detecting the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution, we found that the expression level of oxytocin increased with time.
User Reviews
UNIQ7b174f808f14d1a3-partinfo-00000000-QINU UNIQ7b174f808f14d1a3-partinfo-00000001-QINU