Difference between revisions of "Part:BBa K3755041"

Revision as of 16:29, 9 October 2021

CMV enhancer+CMV promoter+GCaMP6m+SV40 PolyA signal

This composite part is constructed to express GCaMP6m in mammalian cells.

Usage and Biology

This part is cloned in pEGFP, that's why CMV enhancer, CMV promoter and SV40 PolyA signal are used. GCaMP6m is expressed in this composite part. GCaMP6m is a genetically encoded calcium indicator. When intracellular calcium concentration increases, emitted light at about 510nm will increase obviously. To see more information about GCaMP6m, please go to view Part:BBa_K3755007. In our part, we used GCaMP6m to test whether calciums flow in cells after mechanical stimulation as we expected.

Experiment and result

Please go to Part:BBa_K3755007 to view the experiments and results of GCaMP6m.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 542
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 683
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1524
Illegal SapI.rc site found at 1430

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-This part is cloned in pEGFP, that's why CMV enhancer, CMV promoter and SV40 PolyA signal are used.  GCaMP6m is expressed in this composte part. GCaMP6m is a genetically encoded calcium indicator. When intracellular calcium concentration increases, emitted light at about 510nm will increase obviously. To see more information about GCaMP6m, please to to view [[Part:BBa_K3755007]]. In our part, we used GCaMP6m to test whether calciums flow in cells after mechanical stimulation as we expected.
+This part is cloned in pEGFP, that's why CMV enhancer, CMV promoter and SV40 PolyA signal are used.  GCaMP6m is expressed in this composite part. GCaMP6m is a genetically encoded calcium indicator. When intracellular calcium concentration increases, emitted light at about 510nm will increase obviously. To see more information about GCaMP6m, please go to view [[Part:BBa_K3755007]]. In our part, we used GCaMP6m to test whether calciums flow in cells after mechanical stimulation as we expected.
 ===Experiment and result===