Difference between revisions of "Part:BBa K4055525"
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An modified T7 phage promoter which can be regulated by the repressor PhlF | An modified T7 phage promoter which can be regulated by the repressor PhlF | ||
| + | <p> | ||
| + | In order to reduce the amount of leakage expression, referring to the structure of BL21 (DE3) +pET28 protein expression system, we hoped to make the T7 promoter and T7RNAP controlled by PhlF at the same time to reduce the leakage activity, so we added PhlO sequence after the T7 promoter. | ||
| + | </p> | ||
| + | |||
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Revision as of 14:26, 9 October 2021
pT7-PhlO
An modified T7 phage promoter which can be regulated by the repressor PhlF
In order to reduce the amount of leakage expression, referring to the structure of BL21 (DE3) +pET28 protein expression system, we hoped to make the T7 promoter and T7RNAP controlled by PhlF at the same time to reduce the leakage activity, so we added PhlO sequence after the T7 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]