Difference between revisions of "Part:BBa K3755029:Design"
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===Source=== | ===Source=== | ||
− | + | From literature of Eugene Kim's team from Washington University in St. Louis. | |
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===References=== | ===References=== |
Revision as of 09:06, 9 October 2021
cfa-c-mfp5 CDS-his tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 355
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a complete CDS for 3*repeat Mfp5 protein expression and it use be uesd with part:BBa_K3755030. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).
Source
From literature of Eugene Kim's team from Washington University in St. Louis.