Difference between revisions of "Part:BBa K3896017"
Line 3: Line 3:
 
<partinfo>BBa_K3896017 short</partinfo>
 
<partinfo>BBa_K3896017 short</partinfo>
  
−
After reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of igem2018 Tsinghua University team!!!!!, the promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtheriy activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
+
After reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of igem2018 Tsinghua-A!!!!!.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
  
 
We first verify the above circuits by artificially adding AHL.
 
We first verify the above circuits by artificially adding AHL.

Revision as of 07:58, 9 October 2021


Plux_HS-LuxI-EGFP

After reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of igem2018 Tsinghua-A!!!!!.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.

We first verify the above circuits by artificially adding AHL.

figure (contrast of AHL and none)

We want to know the concentration of AHL and set a series of gradients. The results are as follows:

figure (result) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400