Difference between revisions of "Part:BBa K3896016"

Revision as of 07:26, 9 October 2021

The gene of recombinant PmrCAB (DPP4) two-component and quorum sensing system

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein Gly260 to Asp330 to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.

In order to improve the sensitivity of detection, we have improved the above parts and added a quorum sensing system (Fig. 1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1900
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3003
Illegal BamHI site found at 2328
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1147
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3896016 short</partinfo>
+We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein Gly260 to Asp330 to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.
+In order to improve the sensitivity of detection, we have improved the above parts and added a quorum sensing system (Fig. 1).