Difference between revisions of "Part:BBa K3726116"
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<partinfo>BBa_K3726116 short</partinfo> | <partinfo>BBa_K3726116 short</partinfo> | ||
| − | This part is a strong constitutive promoter for cyanobacteria. It is a modified version of PpsbA* promoter originally documented by Wang et al. (B. Wang, C. Eckert, P. Maness and J. Yu, "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803", 2021). | + | This part is a strong constitutive promoter for cyanobacteria. It is a modified version of PpsbA* promoter originally documented by Wang et al. (B. Wang, C. Eckert, P. Maness and J. Yu, "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the <i>Cyanobacterium Synechocystis sp. PCC 6803</i>", 2021). |
The PpsbA-RiboJ includes an insulator sequence after the promoter region. The RiboJ insulator (BBa_K1679038) has been included. This incorporation helps buffering the genetic context effect of the upstream promoter sequence in the design of synthetic circuits. Likewise, the addition of RiboJ has shown to enhance expression levels, becoming this promoter stronger than PpsbA* itself. | The PpsbA-RiboJ includes an insulator sequence after the promoter region. The RiboJ insulator (BBa_K1679038) has been included. This incorporation helps buffering the genetic context effect of the upstream promoter sequence in the design of synthetic circuits. Likewise, the addition of RiboJ has shown to enhance expression levels, becoming this promoter stronger than PpsbA* itself. | ||
| − | PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from Amaranthus hybridus and the Cu2+ inducible promoter petE from Synechocystis PCC6803 has been combined. | + | PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from <i>Amaranthus hybridus</i> and the Cu2+ inducible promoter petE from <i>Synechocystis PCC6803</i> has been combined. |
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | ||
Latest revision as of 23:59, 8 October 2021
P_(PpsbA*-RiboJ)
This part is a strong constitutive promoter for cyanobacteria. It is a modified version of PpsbA* promoter originally documented by Wang et al. (B. Wang, C. Eckert, P. Maness and J. Yu, "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803", 2021).
The PpsbA-RiboJ includes an insulator sequence after the promoter region. The RiboJ insulator (BBa_K1679038) has been included. This incorporation helps buffering the genetic context effect of the upstream promoter sequence in the design of synthetic circuits. Likewise, the addition of RiboJ has shown to enhance expression levels, becoming this promoter stronger than PpsbA* itself.
PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from Amaranthus hybridus and the Cu2+ inducible promoter petE from Synechocystis PCC6803 has been combined.
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 234
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 234
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 234
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 234
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 234
- 1000COMPATIBLE WITH RFC[1000]