Difference between revisions of "Part:BBa K3726116:Design"

Latest revision as of 23:58, 8 October 2021

P_(PpsbA*-RiboJ)

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 234
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 234
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 234
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 234
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 234
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

The PpsbA-RiboJ includes two parts, PpsbA* and RiboJ insulator. PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from Amaranthus hybridus and the Cu2+ inducible promoter petE from Synechocystis PCC6803 has been combined.

References

B. Wang, C. Eckert, P. Maness and J. Yu, "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803", 2021.

P. Till, J. Toepel, B. Bühler, R. Mach and A. Mach-Aigner, "Regulatory systems for gene expression control in cyanobacteria", 2021. .

@@ Line 11: / Line 11: @@
 ===Source===
-The PpsbA-RiboJ includes two parts, PpsbA* and RiboJ insulator. PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from <i>Amaranthus hybridus</i> and the Cu2+ inducible promoter petE from <i><Synechocystis PCC6803</i> has been combined.
+The PpsbA-RiboJ includes two parts, PpsbA* and RiboJ insulator. PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from <i>Amaranthus hybridus</i> and the Cu2+ inducible promoter petE from <i>Synechocystis PCC6803</i> has been combined.
 ===References===