Difference between revisions of "Part:BBa K3726084:Design"

 
 
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<partinfo>BBa_K3726084 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3726084 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.  
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.  
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===Source===
 
===Source===
  
 
The promoter sequence is composed by the fusion of a truncated variant of the rRNA promoter PR from <i>Synechococcus PCC7942</i> and the PS promoter  from <i>E. coli</i>.
 
The promoter sequence is composed by the fusion of a truncated variant of the rRNA promoter PR from <i>Synechococcus PCC7942</i> and the PS promoter  from <i>E. coli</i>.
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===References===
 
===References===
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W. Chungjatupornchai and S. Fa-aroonsawat, "The rrnA promoter as a tool for the improved expression of heterologous genes in cyanobacteria", 2021.

Latest revision as of 23:04, 8 October 2021


P_PS-PR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

The promoter sequence is composed by the fusion of a truncated variant of the rRNA promoter PR from Synechococcus PCC7942 and the PS promoter from E. coli.

References

W. Chungjatupornchai and S. Fa-aroonsawat, "The rrnA promoter as a tool for the improved expression of heterologous genes in cyanobacteria", 2021.