Difference between revisions of "Part:BBa K3726081:Design"
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===Design Notes=== | ===Design Notes=== | ||
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines. | The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | H. Huang, D. Camsund, P. Lindblad and T. Heidorn, "Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology", 2021. | ||
Latest revision as of 19:37, 8 October 2021
P_Ptrc20
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.
Source
Ptrc2O is a modified version of Ptrc1O which is a hybrid between Escherichia coli trp promoter and lacUV5 promoter.
References
H. Huang, D. Camsund, P. Lindblad and T. Heidorn, "Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology", 2021.