Difference between revisions of "Part:BBa K3726080"

Revision as of 19:22, 8 October 2021

P_PrbcL-m36

This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native PCC11801 phycocyanin β-1 subunit promoter.

Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli.

The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR.

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 225
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 225
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 225
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 225
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3726080 short</partinfo>
-This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native Synechococcus PCC11801 phycocyanin &#946;-1 subunit promoter.
+This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native <i>PCC11801</i> phycocyanin &#946;-1 subunit promoter.
-Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli.
+Despite being a cyanobacterial promoter, it has shown high expression levels also in <i>E. Coli</i>.
 The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR.