Difference between revisions of "Part:BBa K3726080:Design"
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===Source=== | ===Source=== | ||
| − | The promoter sequence corresponds with a modified version of the native Synechococcus PCC11801 phycocyanin β-1 subunit promoter. | + | The promoter sequence corresponds with a modified version of the native <i>Synechococcus PCC11801</i> phycocyanin β-1 subunit promoter. |
===References=== | ===References=== | ||
"A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152. | "A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152. | ||
Latest revision as of 19:21, 8 October 2021
P_PrbcL-m36
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 225
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 225
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 225
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 225
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.
Source
The promoter sequence corresponds with a modified version of the native Synechococcus PCC11801 phycocyanin β-1 subunit promoter.
References
"A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.