Difference between revisions of "Part:BBa K3726080:Design"

 
Line 4: Line 4:
  
 
<partinfo>BBa_K3726080 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3726080 SequenceAndFeatures</partinfo>
−
 
  
 
===Design Notes===
 
===Design Notes===
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.  
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.  
−
 
−
 
−
 
  
 
===Source===
 
===Source===
Line 17: Line 13:
  
 
===References===
 
===References===
 +
"A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.

Revision as of 19:20, 8 October 2021


P_PrbcL-m36


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

The promoter sequence corresponds with a modified version of the native Synechococcus PCC11801 phycocyanin β-1 subunit promoter.

References

"A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.