Difference between revisions of "Part:BBa K3726088"

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<partinfo>BBa_K3726088 short</partinfo>
 
<partinfo>BBa_K3726088 short</partinfo>
  
This part corresponds with a Strong Ribosome Binding Site sequence in-silico design to optimize the expression of the enzyme NphT7 encoded by the CDS BBa_K3726000.  
+
This part corresponds with a Strong Ribosome Binding Site sequence in-silico designed to optimize the expression of the enzyme NphT7 encoded by the CDS BBa_K3726000.  
  
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.  
 
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
  
 
<!-- -->
 
<!-- -->
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><b>Sequence & Features</b></span>
<partinfo>BBa_K3726088 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3726000 SequenceAndFeatures</partinfo>
  
  

Revision as of 17:36, 8 October 2021


RBS_NphT7

This part corresponds with a Strong Ribosome Binding Site sequence in-silico designed to optimize the expression of the enzyme NphT7 encoded by the CDS BBa_K3726000.

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.


Sequence & Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 511
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]