Difference between revisions of "Part:BBa K3726088"
LuciaTapia (Talk | contribs) |
LuciaTapia (Talk | contribs) |
||
Line 3: | Line 3: | ||
<partinfo>BBa_K3726088 short</partinfo> | <partinfo>BBa_K3726088 short</partinfo> | ||
− | This part corresponds with a Strong Ribosome Binding Site sequence in-silico | + | This part corresponds with a Strong Ribosome Binding Site sequence in-silico designed to optimize the expression of the enzyme NphT7 encoded by the CDS BBa_K3726000. |
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. | ||
Line 11: | Line 11: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence | + | <span class='h3bb'><b>Sequence & Features</b></span> |
− | <partinfo> | + | <partinfo>BBa_K3726000 SequenceAndFeatures</partinfo> |
Revision as of 17:36, 8 October 2021
RBS_NphT7
This part corresponds with a Strong Ribosome Binding Site sequence in-silico designed to optimize the expression of the enzyme NphT7 encoded by the CDS BBa_K3726000.
The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.
Sequence & Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 461
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 511
Illegal AgeI site found at 883 - 1000COMPATIBLE WITH RFC[1000]