Difference between revisions of "Part:BBa K3726093:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The RBS was designed via an analysis of the RBS sequences of all coding genes longer than 100 amino acids in Synechocystis PCC 6803. It was found that only 26% of the genes contained the core SD sequence, while in E. coli it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of Synechocystis PCC 6803 has been designed. | + | The RBS was designed via an analysis of the RBS sequences of all coding genes longer than 100 amino acids in <i>Synechocystis PCC 6803</i>. It was found that only 26% of the genes contained the core SD sequence, while in <i>E. coli</i> it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of <i>Synechocystis PCC 6803</i> has been designed. |
===Source=== | ===Source=== | ||
− | Coding sequence of this RBS has been found within the genome of Synechocystis PCC 6803. | + | Coding sequence of this RBS has been found within the genome of <i>Synechocystis PCC 6803</i>. |
+ | |||
===References=== | ===References=== |
Revision as of 15:35, 8 October 2021
RBS*
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS was designed via an analysis of the RBS sequences of all coding genes longer than 100 amino acids in Synechocystis PCC 6803. It was found that only 26% of the genes contained the core SD sequence, while in E. coli it was 57% (Ma et al., 2002). Based on that the consensu RBS sequence TAGTGGAGGT which is complementary to the anti-SD sequence AUCACCUCCUUU of Synechocystis PCC 6803 has been designed.
Source
Coding sequence of this RBS has been found within the genome of Synechocystis PCC 6803.
References
Fuentes: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181335/ https://www.sciencedirect.com/science/article/abs/pii/B978012385075100024X?via%3Dihub