Difference between revisions of "Part:BBa K3726107:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON  part the upstream overhang is CGCT, while the downstream overhang is AGCT.
  
  
  
 
===Source===
 
===Source===
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Homology connector has been found within the genome of Synechococcus elongatus PCC 11801.
  
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Genome available at https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2219813
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===References===
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Latest revision as of 14:34, 8 October 2021


3CON5(H)_nNS2-down (PCC 11801)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 646
    Illegal PstI site found at 125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 646
    Illegal PstI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 646
    Illegal XhoI site found at 371
    Illegal XhoI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 646
    Illegal PstI site found at 125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 646
    Illegal PstI site found at 125
    Illegal NgoMIV site found at 396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON part the upstream overhang is CGCT, while the downstream overhang is AGCT.


Source

Homology connector has been found within the genome of Synechococcus elongatus PCC 11801.

Genome available at https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2219813