Difference between revisions of "Part:BBa K3970013"
 
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GAL1 is a commonly used inducible promoter in S. cerevisiae. The transcription of GAL genes involved in galactose metabolism in S. cerevisiae is tightly regulated, and they are not expressed in the absence of galactose. In the presence of galactose, the expression level of galactose was increased by about 1 ,000 fold. A large number of biochemical genetic analyses have preliminarily established the basic regulatory model of GAL gene expression. In addition, the expression of the GAL gene is also regulated by metabolite repression, thus forming a complex regulatory network system.
 
GAL1 is a commonly used inducible promoter in S. cerevisiae. The transcription of GAL genes involved in galactose metabolism in S. cerevisiae is tightly regulated, and they are not expressed in the absence of galactose. In the presence of galactose, the expression level of galactose was increased by about 1 ,000 fold. A large number of biochemical genetic analyses have preliminarily established the basic regulatory model of GAL gene expression. In addition, the expression of the GAL gene is also regulated by metabolite repression, thus forming a complex regulatory network system.
  
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==Sequence==
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<html><p style="word-wrap: break-word;">ATGACTAAATCTCATTCAGAAGAAGTGATTGTACCTGAGTTCAATTCTAGCGCAAAGGAATTACCAAGACCATTGGCCGAAAAGTGCCCGAGCATAATTAAGAAATTTATAAGCGCTTATGATGCTAAACCGGATTTTGTTGCTAGATCGCCTGGTAGAGTCAATCTAATTGGTGAACATATTGATTATTGTGACTTCTCGGTTTTACCTTTAGCTATTGATTTTGATATGCTTTGCGCCGTCAAAGTTTTGAACGAGAAAAATCCATCCATTACCTTAATAAATGCTGATCCCAAATTTGCTCAAAGGAAGTTCGATTTGCCGTTGGACGGTTCTTATGTCACAATTGATCCTTCTGTGTCGGACTGGTCTAATTACTTTAAATGTGGTCTCCATGTTGCTCACTCTTTTCTAAAGAAACTTGCACCGGAAAGGTTTGCCAGTGCTCCTCTGGCCGGGCTGCAAGTCTTCTGTGAGGGTGATGTACCAACTGGCAGTGGATTGTCTTCTTCGGCCGCATTCATTTGTGCCGTTGCTTTAGCTGTTGTTAAAGCGAATATGGGCCCTGGTTATCATATGTCCAAGCAAAATTTAATGCGTATTACGGTCGTTGCAGAACATTATGTTGGTGTTAACAATGGCGGTATGGATCAGGCTGCCTCTGTTTGCGGTGAGGAAGATCATGCTCTATACGTTGAGTTCAAACCGCAGTTGAAGGCTACTCCGTTTAAATTTCCGCAATTAAAAAACCATGAAATTAGCTTTGTTATTGCGAACACCCTTGTTGTATCTAACAAGTTTGAAACCGCCCCAACCAACTATAATTTAAGAGTGGTAGAAGTCACTACAGCTGCAAATGTTTTAGCTGCCACGTACGGTGTTGTTTTACTTTCTGGAAAAGAAGGATCGAGCACGAATAAAGGTAATCTAAGAGATTTCATGAACGTTTATTATGCCAGATATCACAACATTTCCACACCCTGGAACGGCGATATTGAATCCGGCATCGAACGGTTAACAAAGATGCTAGTACTAGTTGAAGAGTCTCTCGCCAATAAGAAACAGGGCTTTAGTGTTGACGATGTCGCACAATCCTTGAATTGTTCTCGCGAAGAATTCACAAGAGACTACTTAACAACATCTCCAGTGAGATTTCAAGTCTTAAAGCTATATCAGAGGGCTAAGCATGTGTATTCTGAATCTTTAAGAGTCTTGAAGGCTGTGAAATTAATGACTACAGCGAGCTTTACTGCCGACGAAGACTTTTTCAAGCAATTTGGTGCCTTGATGAACGAGTCTCAAGCTTCTTGCGATAAACTTTACGAATGTTCTTGTCCAGAGATTGACAAAATTTGTTCCATTGCTTTGTCAAATGGATCATATGGTTCCCGTTTGACCGGAGCTGGCTGGGGTGGTTGTACTGTTCACTTGGTTCCAGGGGGCCCAAATGGCAACATAGAAAAGGTAAAAGAAGCCCTTGCCAATGAGTTCTACAAGGTCAAGTACCCTAAGATCACTGATGCTGAGCTAGAAAATGCTATCATCGTCTCTAAACCAGCATTGGGCAGCTGTCTATATGAATTATAA</p><br></html>
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Latest revision as of 12:33, 8 October 2021


pGAL1

Usage and Biology

GAL1 is a commonly used inducible promoter in S. cerevisiae. The transcription of GAL genes involved in galactose metabolism in S. cerevisiae is tightly regulated, and they are not expressed in the absence of galactose. In the presence of galactose, the expression level of galactose was increased by about 1 ,000 fold. A large number of biochemical genetic analyses have preliminarily established the basic regulatory model of GAL gene expression. In addition, the expression of the GAL gene is also regulated by metabolite repression, thus forming a complex regulatory network system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]