Difference between revisions of "Part:BBa K3970003"
 
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In yeast, the LEU2 gene encodes a β-propyl malate dehydrogenase, which catalyzes the third step of leucine biosynthesis, the conversion from β-isopropyl malate to α-ketoishaproic acid. Because β-isopropyl malate dehydrogenase (EC 1.1.1.85) is quite conserved in its regulatory mechanism and primary structure, LEU2 gene is a useful selection marker in a variety of yeast.
 
In yeast, the LEU2 gene encodes a β-propyl malate dehydrogenase, which catalyzes the third step of leucine biosynthesis, the conversion from β-isopropyl malate to α-ketoishaproic acid. Because β-isopropyl malate dehydrogenase (EC 1.1.1.85) is quite conserved in its regulatory mechanism and primary structure, LEU2 gene is a useful selection marker in a variety of yeast.
  
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==Sequence==
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:29, 8 October 2021


pLEU2-LEU2

Usage and Biology

In yeast, the LEU2 gene encodes a β-propyl malate dehydrogenase, which catalyzes the third step of leucine biosynthesis, the conversion from β-isopropyl malate to α-ketoishaproic acid. Because β-isopropyl malate dehydrogenase (EC 1.1.1.85) is quite conserved in its regulatory mechanism and primary structure, LEU2 gene is a useful selection marker in a variety of yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]