Difference between revisions of "Part:BBa K3726104"

Revision as of 16:20, 7 October 2021

5CON1(H)_NS1(mod)-up (PCC 11801)

This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.

Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).

Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.

Sequence & Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 18
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 18
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 18
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 18
Illegal AgeI site found at 594
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
-<partinfo>Bba_K3726094 short</partinfo>
+<partinfo>Bba_K3726104 short</partinfo>
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 <span class='h3bb'><b>Sequence & Features</b></span>
-<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K3726104 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K3726094 parameters</partinfo>
+<partinfo>BBa_K3726104 parameters</partinfo>
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