Difference between revisions of "Part:BBa K3726065:Design"
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
This part corresponds with the codon optimized coding sequence of AcrbV2. | This part corresponds with the codon optimized coding sequence of AcrbV2. | ||
| − | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. | + | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i> . |
In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases | In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases | ||
| Line 17: | Line 17: | ||
===References=== | ===References=== | ||
| − | "Enhancing Tolerance to Short-Chain Alcohols by Engineering the Escherichia coli AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol", ACS Publications, 2021. [Online]. Available: https://pubs.acs.org/doi/10.1021/sb400065q. | + | "Enhancing Tolerance to Short-Chain Alcohols by Engineering the <i>Escherichia coli</i> AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol", ACS Publications, 2021. [Online]. Available: https://pubs.acs.org/doi/10.1021/sb400065q. |
Latest revision as of 15:35, 7 October 2021
CDS_AcrbV2
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 331
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 60
Illegal PstI site found at 331 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 331
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 331
Illegal NgoMIV site found at 1467
Illegal NgoMIV site found at 2983 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of AcrbV2. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942 . In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases
Source
Coding sequence of this membrane transporter subunit comes from directed evolution of AcrB Escherichia coli (strain K12) gen. Uniprot reference: https://www.uniprot.org/uniprot/P31224
References
"Enhancing Tolerance to Short-Chain Alcohols by Engineering the Escherichia coli AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol", ACS Publications, 2021. [Online]. Available: https://pubs.acs.org/doi/10.1021/sb400065q.