Difference between revisions of "Part:BBa K3726035:Design"

Revision as of 21:44, 6 October 2021

CDS_mtkB

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 279
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 279
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 94
Illegal BamHI site found at 552
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 279
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 279
Illegal NgoMIV site found at 715
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part has been designed to be assembled within the part "BBa_K3726038" thought Gibson.

Source

Coding sequence of this Malate--CoA ligase subunit alpha has been obtained from Methylococcus Capsulatus genome thought PCR. Uniprot reference:https://www.uniprot.org/uniprot/P53595

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.

@@ Line 4: / Line 4: @@
 <partinfo>BBa_K3726035 SequenceAndFeatures</partinfo>
 ===Design Notes===
 This part has been designed to be assembled within the part "BBa_K3726038" thought Gibson.
 ===Source===
-Coding sequence of this Malate--CoA ligase subunit alpha has been obtained from Methylococcus Capsulatus genome thought PCR.
+Coding sequence of this Malate--CoA ligase subunit alpha has been obtained from <i>Methylococcus Capsulatus</i>  genome thought PCR.
 Uniprot reference:https://www.uniprot.org/uniprot/P53595
 ===References===
+H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.