Difference between revisions of "Part:BBa K4055537"

 
 
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<partinfo>BBa_K4055537 short</partinfo>
 
<partinfo>BBa_K4055537 short</partinfo>
  
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This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter.
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This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter.In fact, there are holes in the system. The system is located on a low copy plasmid, while the plasmid expressing the protein is a high copy plasmid. So the protein can leak out. The system does not achieve what we are looking for
  
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===Experiment===
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Wrap it in black paper, cut out a specific pattern and illuminate it with blue light. After a few days the proteins were observed to be expressed in a specific pattern. Unfortunately, the protein was not expressed in sufficient concentrations and leakage occurred
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4055537 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4055537 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:13, 6 October 2021


BlueLight_inducing_T7RNAPolymerase

This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter.In fact, there are holes in the system. The system is located on a low copy plasmid, while the plasmid expressing the protein is a high copy plasmid. So the protein can leak out. The system does not achieve what we are looking for

Experiment

Wrap it in black paper, cut out a specific pattern and illuminate it with blue light. After a few days the proteins were observed to be expressed in a specific pattern. Unfortunately, the protein was not expressed in sufficient concentrations and leakage occurred

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 372
    Illegal NgoMIV site found at 466
    Illegal NgoMIV site found at 598
    Illegal NgoMIV site found at 703
    Illegal NgoMIV site found at 996
    Illegal NgoMIV site found at 1090
    Illegal AgeI site found at 247
    Illegal AgeI site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 136
    Illegal BsaI site found at 760