Difference between revisions of "Part:BBa K4055533"
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Through the combination of three components, we made a blue light photosensitive system. When exposed to blue light,this part can activate the following T7 RNA polymerase.  
 
Through the combination of three components, we made a blue light photosensitive system. When exposed to blue light,this part can activate the following T7 RNA polymerase.  
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<p>
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In particular,in this circuit, the expression of histidinine kinase YF1 and its homologous regulatory factor FixJ are tightly regulated by LacIq promoter, while the expression repressor cI of phage λ is controlled by pFixK2 promoter. Under blue light irradiation, the kinase activity and subsequent cI expression of YF1 were inhibited, which then activated the λ promoter pR and promoted the expression of CSGA-MFP3S-PEP.
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</p>
  
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===Experiment===
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Experiments in BBa_K4055535 are used to prove the effectiveness of the system
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4055533 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4055533 SequenceAndFeatures</partinfo>
  

Revision as of 15:53, 6 October 2021


CsgA-Mfp3S-pep

Through the combination of three components, we made a blue light photosensitive system. When exposed to blue light,this part can activate the following T7 RNA polymerase.

In particular,in this circuit, the expression of histidinine kinase YF1 and its homologous regulatory factor FixJ are tightly regulated by LacIq promoter, while the expression repressor cI of phage λ is controlled by pFixK2 promoter. Under blue light irradiation, the kinase activity and subsequent cI expression of YF1 were inhibited, which then activated the λ promoter pR and promoted the expression of CSGA-MFP3S-PEP.

Experiment

Experiments in BBa_K4055535 are used to prove the effectiveness of the system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]