Difference between revisions of "Part:BBa K3838888"

 
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Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from LACTObacillus casei Lc18.
 
Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from LACTObacillus casei Lc18.
 
In a team project, SOD related parts are composed as follows:
 
In a team project, SOD related parts are composed as follows:
−
J23100 promoter +RBS+SOD+ HIS tag +T7 terminator
+
J23100 promoter +RBS+SOD+ HIS tag +Signal peptide+T7 terminator
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:49, 6 October 2021


J-SOD

The overproduction of Superoxide dismutase (SOD) and reactive oxygen species (ROS) is considered to be one of the pathogenesis of IBD. The use of SOD can significantly reduce the peroxidation reaction in the inflamed colon. Studies have shown that the decreased expression of Cu/Zn-SOD has been observed in IBD patients. SOD can not be directly treated, so carrier transport is needed. Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from LACTObacillus casei Lc18. In a team project, SOD related parts are composed as follows: J23100 promoter +RBS+SOD+ HIS tag +Signal peptide+T7 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 394
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 394
    Illegal BglII site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 394
    Illegal NgoMIV site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]