Difference between revisions of "Part:BBa K3726104"

 
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<partinfo>BBa_K3726104 short</partinfo>
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<partinfo>Bba_K3726094 short</partinfo>
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This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.
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Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the  level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).
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Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.
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The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit. 
  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence & Features</b></span>
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<partinfo>BBa_K3726104 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3726104 parameters</partinfo>
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<partinfo>BBa_K3726094 parameters</partinfo>
 
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Revision as of 11:16, 6 October 2021


T(5a)_Teck


This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.

Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).

Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]