Difference between revisions of "Part:BBa K3726094:Design"

 
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===Design Notes===
 
===Design Notes===
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.
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This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.
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===References===
 
===References===
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Revision as of 10:51, 6 October 2021


T(5a)_Teck


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.

This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.



Source

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References

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