Difference between revisions of "Part:BBa K3726005:Design"

 
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===References===
 
===References===
 +
R. Miao, X. Liu, E. Englund, P. Lindberg and P. Lindblad, "Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases", 2021.

Revision as of 16:56, 4 October 2021


CDS_Slr1192


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
    Illegal BglII site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of Slr1192 enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases



Source

Coding sequence of this Aldehyde reductase has been found within the genome of Synechocystis PC 6803. Uniprot reference: https://www.uniprot.org/uniprot/P74721


References

R. Miao, X. Liu, E. Englund, P. Lindberg and P. Lindblad, "Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases", 2021.