Difference between revisions of "Part:BBa K3934015"
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<partinfo>BBa_K3934015 short</partinfo> | <partinfo>BBa_K3934015 short</partinfo> | ||
| − | A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC). | + | A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC) [1]. |
===Sequence and Features=== | ===Sequence and Features=== | ||
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<partinfo>BBa_K3934015 parameters</partinfo> | <partinfo>BBa_K3934015 parameters</partinfo> | ||
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| + | Sources: | ||
| + | <br>[1] W. T. Booth et al., ‘Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability’, ACS Omega, vol. 3, no. 1, pp. 760–768, Jan. 2018, doi: 10.1021/acsomega.7b01598. | ||
Revision as of 10:03, 4 October 2021
6xHis-Tag for protein purification
A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC) [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sources:
[1] W. T. Booth et al., ‘Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability’, ACS Omega, vol. 3, no. 1, pp. 760–768, Jan. 2018, doi: 10.1021/acsomega.7b01598.