Difference between revisions of "Part:BBa K3804009"

Latest revision as of 06:40, 3 October 2021

Reporter for quorum sensing systems in yeast with qscR

We created this part with reference to BBa_K1024002 in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator　and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1673
Illegal PstI site found at 2036
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1673
Illegal PstI site found at 2036
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1673
Illegal XhoI site found at 1595
Illegal XhoI site found at 1646
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1673
Illegal PstI site found at 2036
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1673
Illegal PstI site found at 2036
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1811

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3804009 short</partinfo>
-We created this　part　with reference to <bbpart>BBa_K1024002</bbpart> in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved <bbpart>BBa_K1024002</bbpart> by adding ADH1 terminator and CYC1terminator　and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.
+We created this part with reference to <bbpart>BBa_K1024002</bbpart> in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved <bbpart>BBa_K1024002</bbpart> by adding ADH1 terminator and CYC1terminator　and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.
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