Difference between revisions of "Part:BBa K3804012"
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<partinfo>BBa_K3804012 short</partinfo> | <partinfo>BBa_K3804012 short</partinfo> | ||
| − | This part aims to secrete HBD3(human β-defensin-2) with yeast. In this part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, high methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when MET17 promoter is activated. | + | This part aims to secrete HBD3(human β-defensin-2) with yeast. In this part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, high methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, this part make yeast secrete HBD3 when MET17 promoter is activated. |
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Revision as of 06:34, 3 October 2021
HBD3 in yeast regulated by MET17p
This part aims to secrete HBD3(human β-defensin-2) with yeast. In this part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, high methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, this part make yeast secrete HBD3 when MET17 promoter is activated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]