Difference between revisions of "Part:BBa K3753000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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It should be synthesized with an optimized codon usage for different species.
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The coding region of petunia PAAS was obtained by PCR amplification using Petunia hybrida genome as template.
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(Gen-Bank accession number DQ243784.1)
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===Source===
 
===Source===

Revision as of 03:36, 3 October 2021


PAAS-Petunia


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding region of petunia PAAS was obtained by PCR amplification using Petunia hybrida genome as template. (Gen-Bank accession number DQ243784.1)

Source

The coding region of petunia PAAS was amplified by PCR using forward and reverse primers (5'-ATTCATATGGATACTATCAAAATCAACCC-3' and 5'-GGGATCCTTCTACGCATTCAGCATCATAG-3', respectively). (Gen-Bank accession number DQ243784.1)


References