Difference between revisions of "Part:BBa K3718002"

Revision as of 16:51, 2 October 2021

Customizable detection and expression with increased growth

Part II of toolkit for Detection with Proliferation Control

The Modular Receptor Platform (MRP) is designed according to the paper published in 2018 (Chang et al, 2018). When the antigen/ligand binds to the nanobody, dimerization of the protein CadC domains occur. As a result, this allows the binding of the pCadBA operon region and transcription downstream is promoted at the same time. The VHH region has high flexibility as it can be customized for binding with different targets, so that any specific targets with single domain antibodies available will be able to bind with the VHH region for detection. Users can add their own VHH region after the transmembrane region DNA via restriction ligation.

For the developers to clone the genes that they want to express, a multiple cloning site (MCS) K660500 is present in the same region as the genes that allow for restored growth so it will be expressed when the antigen is detected. The MCS allows for the insertion of the target genes to be less complex and be less time consuming.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 67
Illegal suffix found in sequence at 629
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 67
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 630
Illegal PstI site found at 644
Illegal NotI site found at 73
Illegal NotI site found at 637
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 67
Illegal BglII site found at 2337
Illegal BamHI site found at 912
Illegal XhoI site found at 906
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 67
Illegal suffix found in sequence at 630
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 67
Illegal XbaI site found at 82
Illegal SpeI site found at 630
Illegal PstI site found at 644
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
-Part II of toolkit for Detection with Proliferation Control
+<p>Part II of toolkit for Detection with Proliferation Control</p>
-The Modular Receptor Platform (MRP) is designed according to the paper published in 2018 (Chang et al, 2018). When the antigen/ligand binds to the nanobody, dimerization of the protein CadC domains occur. As a result, this allows the binding of the pCadBA operon region and transcription downstream is promoted at the same time. The VHH region has high flexibility as it can be customized for binding with different targets, so that any specific targets with single domain antibodies available will be able to bind with the VHH region for detection. Users can add their own VHH region after the transmembrane region DNA via restriction ligation.
+<p>The Modular Receptor Platform (MRP) is designed according to the paper published in 2018 (Chang et al, 2018). When the antigen/ligand binds to the nanobody, dimerization of the protein CadC domains occur. As a result, this allows the binding of the pCadBA operon region and transcription downstream is promoted at the same time. The VHH region has high flexibility as it can be customized for binding with different targets, so that any specific targets with single domain antibodies available will be able to bind with the VHH region for detection. Users can add their own VHH region after the transmembrane region DNA via restriction ligation.</p>
-For the developers to clone the genes that they want to express, a multiple cloning site (MCS) K660500 is present in the same region as the genes that allow for restored growth so it will be expressed when the antigen is detected. The MCS allows for the insertion of the target genes to be less complex and be less time consuming.
+<p>For the developers to clone the genes that they want to express, a multiple cloning site (MCS) K660500 is present in the same region as the genes that allow for restored growth so it will be expressed when the antigen is detected. The MCS allows for the insertion of the target genes to be less complex and be less time consuming.</p>