Difference between revisions of "Part:BBa K3787000"
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<partinfo>BBa_K3787000 short</partinfo> | <partinfo>BBa_K3787000 short</partinfo> | ||
| − | + | This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, PETase and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker. | |
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Latest revision as of 12:05, 2 October 2021
PETase(WT)-MHETase
This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, PETase and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]