Difference between revisions of "Part:BBa K3731004:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None.
+
PCR-amplified CFppk1-vgb-mazE was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.
−
 
+
  
  

Latest revision as of 02:02, 1 October 2021


EPVM-PBBR1MCS-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5166
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5166
    Illegal XbaI site found at 5181
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR-amplified CFppk1-vgb-mazE was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.


Source

For the construction of EPVM-PBBR1MCS-2, genomic DNA of E.Coli BL21 was used as the template to PCR-amplify ppk1, vgb and mazE with primers.

References