Difference between revisions of "Part:BBa K3718004"
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Single guide RNA targeting iclR, for knock-out. | Single guide RNA targeting iclR, for knock-out. | ||
| − | < | + | <h1>crRNA</h1> |
Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.4, which is positioned at the beginning of the iclR gene, to maximize iclR disfunctioning. The crRNA has a desirable CG content at 50% and its efficiency is predicted at 70.41%. | Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.4, which is positioned at the beginning of the iclR gene, to maximize iclR disfunctioning. The crRNA has a desirable CG content at 50% and its efficiency is predicted at 70.41%. | ||
| − | < | + | <h1>tracrRNA and T7 terminator</h1> |
The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006. | The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006. | ||
Revision as of 14:26, 30 September 2021
sgRNA for iclR with terminator - transcription template
Single guide RNA targeting iclR, for knock-out.
crRNA
Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.4, which is positioned at the beginning of the iclR gene, to maximize iclR disfunctioning. The crRNA has a desirable CG content at 50% and its efficiency is predicted at 70.41%.
tracrRNA and T7 terminator
The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 171 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 172
Illegal PstI site found at 186
Illegal NotI site found at 7
Illegal NotI site found at 179 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 172 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 172
Illegal PstI site found at 186 - 1000COMPATIBLE WITH RFC[1000]