Difference between revisions of "Part:BBa K3804012"
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<partinfo>BBa_K3804012 short</partinfo>
 
<partinfo>BBa_K3804012 short</partinfo>
  
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This part aims to secrete HBD3(human β-defensin-2) with yeast. In the part, we used MET17promoter because the previous study shows that using MET17 promoter improves secretion of HBD2(Møller et al. 2017). By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when MET17 promoter is activated.  
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This part aims to secrete HBD3(human β-defensin-2) with yeast. In the part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when MET17 promoter is activated.  
  
 
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Revision as of 14:01, 30 September 2021


HBD3 in yeast regulated by MET17p

This part aims to secrete HBD3(human β-defensin-2) with yeast. In the part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when MET17 promoter is activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]