Difference between revisions of "Part:BBa K3866033"

Revision as of 10:43, 30 September 2021

pAndersonJ23115:RBS-bglX-terminator

Bglx BBa_K523002 under control of a constitutive promoter BBa_K3505012.

Fig.1: ΒglX under control of a constitutive promoter.

Usage and Biology

Τhis part consists of AndersonJ23115 promoter BBa_K3505012, bglx BBa_K523002 and Double Terminator BBa_K3505017. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx is constitutively expressed. The product protein is expected to be in the periplasm.

Design Notes

Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008and has overhangs compatible for GoldenBraid cloning.

Experimental Use and Experience

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 62
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1721
Illegal AgeI site found at 1943
Illegal AgeI site found at 2132
1000
COMPATIBLE WITH RFC[1000]

Source

Synthesized by IDT.

@@ Line 15: / Line 15: @@
 ===Design Notes===
 Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart>and has overhangs compatible for GoldenBraid cloning.
+===Experimental Use and Experience===
 ===Sequence and Features===