Difference between revisions of "Part:BBa K3804008"

 
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<partinfo>BBa_K3804008 short</partinfo>
 
<partinfo>BBa_K3804008 short</partinfo>
  
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This part expresses LasI. LasI is a protein which synthesize AHL.
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This part expresses LasI. LasI is a protein which synthesize 3-oxo-C12-AHL. AHL is used for quorum sensing system in Pseudomonas aeruginosa. It bind with LasR and forms a complex(AHL-LasR). Two AHL-LasR form a dimer and activate Las promoter. In our project, We used this part in order to express AHL in yeast.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:03, 30 September 2021


LasI

This part expresses LasI. LasI is a protein which synthesize 3-oxo-C12-AHL. AHL is used for quorum sensing system in Pseudomonas aeruginosa. It bind with LasR and forms a complex(AHL-LasR). Two AHL-LasR form a dimer and activate Las promoter. In our project, We used this part in order to express AHL in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 270
  • 1000
    COMPATIBLE WITH RFC[1000]